Preliminary Investigation of the Effect of Chemical Sanitizers and UV-C Light on Listeria monocytogenes Biofilm Survivability

Ellen A. Mendez, Brian Tande, Jessie Vipham, Valentina Trinetta Biblographic citation: Food Protection Trends, vol. 42, no. 4, pp. 278-283, Jul 2022 Volume 42, Issue 4: Pages 278–283 DOI: 10.4315/FPT-21-025

The ability of Listeria monocytogenes to adapt under different environments and form biofilms is a challenge for food safety. Mature biofilms are difficult to disrupt. Chemical sanitizers combined with nonthermal technol-ogies might be an effective way to control L. monocytogenes biofilms. This study was conducted to investigate L. monocytogenes biofilm survival after treatments with chemical sanitizers and UV-C light alone or in combi-nation. A Centers for Diseases Control and Prevention biofilm reactor was used to grow 4-day-old multistrain L. monocytogenes biofilms on stainless steel. Biofilm survival was evaluated after 10 min of exposure to lactic acid (4%), peroxy acid (100 ppm), and quaternary ammonium (400 ppm) alone or in combination with 15 or 30 min of exposure to UV-C light (254 nm). The sequen-tial treatment effect was also evaluated. Reductions of 2.6 to 3.6 log CFU/cm2 were observed with chemical sanitizers, whereas a maximum of 1.8 log CFU/cm2 reduction was recorded after UV-C treatment. Combined treatments had an enhanced effect, and the sequence of antimicrobial treatments was significant for lactic acid and peroxy acid (P < 0.05). The results obtained in this research offer an initial understanding of the response of L. monocytogenes biofilm to chemical sanitizers and con-tribute to development of effective strategies to control this pathogen in the food processing environment.

Subscribe Today

Subscribe to Food Protection Trends to stay up to date on the information you need, including scientific research and articles reporting on a variety of food safety and quality topics.

Request Permission to Reuse Content

This link will take you to the Copyright Clearance Center where you can submit a request to reuse IAFP’s content found in our publications. Please note that no part of any publications may be reproduced or transmitted in any form without prior permission from IAFP.