Multiple Rapid Methods for Identifying Poultry Samples Exceeding a Salmonella Threshold Level
Mar 4, 2025 (4:00 PM - 5:00 PM Eastern Time)
Webinar Description
New strategies to enhance control of Salmonella in meat and poultry include identifying product that exceeds various Salmonella levels. Several rapid methods are available for identifying samples meet or exceed a Salmonella threshold including an abbreviated enrichment followed by real time PCR (rtPCR) quantification, concentration of the sample followed by rtPCR quantification, and enrichment of sample aliquots informed by a modification of the Poisson Probability Distribution. This webinar will discuss the abilities of Most Probable Number (MPN), Hygiena BAX SalQuant (abbreviated enrichment followed by rtPCR), BioMerieux GENE-UP QUANT (sample concentration followed by rtPCR), and Poisson Probability Distribution based threshold methods for identifying poultry samples meeting or exceeding a Salmonella threshold level. Learning Objectives: • Understand the limitations of the traditional “gold standard” bacterial quantification Most Probable Number Method. • Understand the factors that may influence results of rapid quantification methods that gauge the post-enrichment Salmonella. • Appreciate the issues that could impact results of rapid bacterial quantification methods that concentrate the sample. • Gain an understanding of novel methods to identify samples with Salmonella concentrations meeting or exceeding a user defined threshold.
Professional Development Groups
- Applied Laboratory Methods Professional Development Group
Presenters
- John Schmidt, Presenter Research Microbiologist U.S. Meat Animal Research Center, Meat Safety and Quality Research Unit·USDA
- Amanda Brookhouser-Sisney, Moderator Sr. Technical Director·Midwest Laboratories